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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. As maturation continues in the granulocytic series the nucleus of the metamyelocyte becomes kidney or bean shaped.
Identify the cell in this illustration indicated by the arrow:

A) Metamyelocyte
B) Myeloblast
C) Monocyte
D) Promonocyte

2. Gram-positive bacteria retain the primary crystal violet stain that is applied in the Gram stain because of the cross-linking of the thick peptidoglycan layer in their cell walls. Their cell walls contain less lipids than that of a gram-negative bacteria, and this reduces the permeability of their cell wall to the organic solvents that are used as a decolorizer. When the counterstain is added, it may enter the gram-positive cell, but does not change the color of the cells.
A thick peptidoglycan is MOST often associated with which of the following types of bacteria?

A) Gram-negative bacteria
B) Both gram-negative and gram-positive bacteria
C) Gram-positive bacteria

3. Once substance H is developed, the addition of the sugar N-acetylgalactosamine to the terminal position of the chain gives the molecule "A" antigenic activity.
Which specific terminal sugar causes a red cell to have A antigenic activity?

A) Galactose plus glucose
B) N-acetylgalactosamine
C) Galactose
D) Glucose

4. Streptococcus pyogenes is the correct answer. Streptococcus pneumoniae is alpha-hemolytic which demonstrate a green area of partial hemolysis around the colonies. Staphylococcus aureus will produce complete hemolysis but is catalase positive. Streptococcus agalactiae is beta-hemolytic but only produces a partial clearing of the red blood cells in sheep blood agar.
Complete hemolysis of sheep blood agar as demonstrated by the image below would be seen in which of the following catalase-negative isolates?

A) Streptococcus agalactiae
B) Streptococcus pneumoniae
C) Staphylococcus aureus
D) Streptococcus pyogenes

5. The term universal precautions refers to:

A) universal blood donors.
B) treating only CERTAIN specimens as infectious.
C) mixing organic chemicals under a hood.
D) wellness clinic available to everyone.

Solutions: