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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A patient with cystic fibrosis experiences a pulmonary exacerbation. Sputum culture grows a mucoid strain of Pseudomonas aeruginosa. The mucoid phenotype is primarily due to the overproduction of:

A) Elastase
B) Exotoxin A
C) Pyocyanin
D) Alginate

2. A research team is investigating the mechanism of bacterial resistance to a novel fluoroquinolone antibiotic.
They isolate a resistant strain of Escherichia coli from a patient with a urinary tract infection. Genetic analysis of this strain reveals a point mutation in the gyrA gene. This mutation MOST likely results in:

A) Modification of the ribosomal target site, preventing antibiotic binding.
B) Production of an enzyme that inactivates the fluoroquinolone molecule.
C) Alteration of the DNA gyrase enzyme, reducing its affinity for the fluoroquinolone.
D) Increased expression of efflux pumps that actively transport the antibiotic out of the cell.

3. A clinical microbiology laboratory is performing quality control on Gram stain reagents. A control slide containing Escherichia coli and Staphylococcus aureus shows both organisms staining purple after the complete staining procedure. The MOST likely cause of this error is:

A) Over-decolorization with alcohol-acetone.
B) Omission of the iodine mordant.
C) Insufficient washing after crystal violet.
D) Insufficient application of crystal violet.

4. A patient presents with a pruritic rash consisting of raised, serpiginous tracks on their skin. The patient recently returned from a Caribbean vacation and reported walking barefoot on the beach. The MOST likely causative organism is:

A) Pediculus humanus
B) Sarcoptes scabiei
C) Larvae of Strongyloides stercoralis
D) Larvae of Ancylostoma braziliense

5. For epidemiological tracking and outbreak investigations of Clostridioides difficile infection (CDI), which molecular typing method based on PCR amplification targeting repetitive elements within the pathogenicity locus (PaLoc) has historically been widely used and designated by specific nomenclature (e.g., NAP1/BI
/027)?

A) Multilocus sequence typing (MLST)
B) Pulsed-field gel electrophoresis (PFGE)
C) Whole-genome sequencing (WGS) SNP analysis
D) PCR Ribotyping

Solutions: